National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Chilli leaf curl disease, caused by the begomovirus (family- Geminiviridae), is an economically important disease of chilli (Capsicum annum L.) that can be very destructive in tropical and subtropical regions. In July- august 2015, upward leaf-curling, distortion, vein yellowing or more generalized leaf yellowing and/or stunted growth with bushy appearance with high abundance of vector whitefly (Bemisia tabaci) observed in chilli plants in the area of Cooch Behar, a diverse agroclimatic zone of sub Himalayan West Bengal, India. The incidence begomovirus infection on chilli was observed up to an extent of 20-90%. Presence of begomovirus infection was confirmed through Polymerase chain reaction (PCR) using begomovirus specific degenerate primer SPG1/SPG2. PCR product of 920 bp was detected, partially sequenced and has been deposited in NCBI (GeneBank Accession No. LT622252). The BLASTn analysis was performed with other begomovirus isolates in the GeneBank database of NCBI. The replication associated protein (AC1) gene of the virus showed highest 94% nucleotide sequence identity (nsi) with both Tomato leaf curl Joydebpur virus clone Moh9, complete genome [GeneBank Acc. No.- KJ028211] and Tomato leaf curl Joydebpur virus clone HJP09 segment DNA-A, complete sequence [GeneBank Acc. No. JQ654463]. This is the first report of a Begomovirus infecting chilli (Capsicum annum L.) in sub-Himalayan regions of West Bengal.
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