National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The aim of this study was produce biosurfactant by Cunninghamella bertholletiae Stadel, grown in low-cost, containing corn steep liquor and post-frying oil, and still checking the influence of agitation in this process. For determination of surface tension the free cells metabolic liquid containing the biosurfactant after 120h of cultivation were measured by an automatic tensiometer using the ring NUOYDU, through its immersion in the liquid, recording the force required to pull it through the air-liquid interface. To determine the rate emulsification, 2.0ml of cell-free metabolic liquid was added to 1.0ml of substrate: kerosene, diesel oil, mineral oil, soybean oil, motor oil and motor oil burned in test tubes, and the mixture was vortex for 2 min at maximum speed. After 24h of rest, the formed emulsions were calculated by the height of the full height of the emulsion mixture multiplied by 100 and expressed as a percentage. The results indicated a reduction in surface tension metabolic liquid, free of cells, 70mN/m, 27, 37mN of water/m2in the condition (4% corn steep liquor and soybean oil post-frying 3%) without stirring. The highest rates of emulsification were obtained with engine oil. However we cannot say that the biosurfactant studied is a good emulsifier.
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