National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The pigment producing bacteria and fungi were isolated from soil, damping site, compost, industrial effluent, spoiled fruits and vegetables. A total of fifteen bacterial and nine fungal pigment-producing isolates were obtained and screened on nutrient agar and potato dextrose medium, respectively. The pigments from selected isolates were extracted by solvent extraction (acetone and methanol). The antimicrobial activity of selected isolates was determined by disc agar diffusion technique against standard isolates of Staphylococcus aureus, Klebsiella pneumonia, Escherichia coli and Fusarium oxysporum. The cytotoxicity was studied by using yeast toxicity test (YTT). Bacterial isolates BP-8 exhibited the inhibitory effect against Saccharomyces cerevisiae and E.coli. The Rf value of pigment extract from different bacterial isolates varied from 0.86 to 0.91 whereas it was 0.92 by fungal isolate FP-6 as determined by thin layer chromatography (TLC). ). The bacterial isolates BP-2 and BP-6 were Gram+ve and BP-8 was Gram-ve. Out of these BP-6 and BP-8 were non spore former. These bacterial isolates belonged to the genus Bacillus, Micrococcus and Serratia. The fungal isolate was identified as Aspergillus sp.
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