National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Culture-independent microbiota is relatively unexplored in Mexican population. The aim of this study was to characterize the microbiota of Mexican young healthy adults by means of traditional culture and metagenomic analysis in order to provide novel insights and have a better understanding about the healthy baselines from which to detect differences associated with diseases. The bacterial microbiota of the nose and pharynx from 75 healthy nonsmoking Mexican young adults was examined by conventional cultures and culture-independent methods. The hypervariable region (V6-V8) of the16S rRNA gene was PCR amplified from isolated DNA and DGGE analyzed, bands excised were sequenced and phylogenetic analysis was done. The study showed that the bacterial microbiota of the pharynx was richer than that of the nose. Using conventional culture methods results showed that gram positive Staphylococcus aureus and S. epidermidis were found in both niches and gram negative bacteria such as Escherichia coli, Klebsiella sp. and Moraxella sp. were the most abundant genera in the nose and Enterobacter sp. in the pharynx. Firmicutes and Proteobacteria phyla accounted for the majority of the bacteria detected in both niches. To our knowledge this is the first report describing partially the composition of and the variability within the nasopharyngeal microbiota of a Mexican young adult population and therefore we will be able to investigate other different cities and compared them to find out where are the highest microbial contaminated zones and take some hygienic measures in accordance to the normativity reports.
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