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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present investigation was carried out to study the influence of antioxidants on in vitro culture of Gliricidia sepium. Soft nodal stem segments were inoculated on MS medium supplemented with desired concentrations plant growth regulator such as cytokinins (BAP/Kinetin) and different antioxidants. Antioxidants viz., activated charcoal, ascorbic acid, citric acid and polyvinylpyrollidone were used to control the accumulation of phenolic compounds in the culture medium and enhance the rate of micropropagation. All cultures were incubated at 25+20C under fluorescent light in a 14: 10 hour’s photoperiod. Maximum number of shoot bud induction was obtained when MS medium is supplemented with 0.5 mg/l and 250 mg/l ascorbic acid.