National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Identification of genes involved in mtDNA maintenance in Caenorhabditis elegans that could represent candidate genes for mitochondrial diseases associated with quantitative or qualitative mtDNA abnormalities was performed using our previous validated method of RNAi combined with ethidium bromide. This was to knock down C. elegans genes homologous to human genes known to be involved in mtDNA stability. Silencing of these candidate genes were repeated again without EtBr to measure the worm’s mtDNA content and the efficiency of gene silencing by Q-PCR and RT-Q-PCR. Among the 182 genes encoding mitochondrial proteins tested randomly, we found that inactivation of 25 of them lead to EtBr sensitivity. Out of these genes, 20 fall into four main categories of metabolism (GOT2, WWOX, ALDH1L2, WWOX, AGMAT and ACOT9), protein synthesis (TRMU, MRPL47, MRPS14, MRPS18C, MRPL4 and MRPL12), mitochondrial morphology (OPA1,) and respiratory chain oxidative phosphorylation (ATP5F1, NDUFS4, FH, CYP2C8, CYP3A7, CYP2C8 and CYP2C8). The human orthologs of these genes as presented in this study could be considered as reservoir of new genes which will represent a helpful tool and knowledge for mitochondrial diseases, as they would be considered as candidate genes for patients.
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