National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The aim of this study was to evaluate the effect of the hormonal treatment during early postpartum period on the uterine involution, the steroid hormone levels and the ovarian activity in Bulgarian Murrah buffaloes. The experiment was carried out with 20 clinically healthy animals in breeding season, randomly allotted in two groups: control group (non treated; n=10) and experimental group (hormonal treated; n=10) received 100 µg GnRH and 25 mg PGF2α seven days apart starting at day 14 after calving. Fertile bulls were ensured for all buffaloes. A transrectal ultrasonographic examination of the uterus and the ovaries and blood samples collection were performed at the days of the treatment. Serum estradiol-17β and progesterone levels were determined by ELISA. Ultrasound pregnancy check was done at day 30 and 60 postpartum. After data processing, mean cervical and gravid uterine horn diameters, involution rate of cervix and gravid uterine horn, percentage of the buffaloes bearing large follicle and/or corpus luteum, mean concentration of steroid hormones and pregnant buffaloes were calculated.
The present study demonstrated significant (P>0.05) decrease of the mean diameters of cervix and gravid uterine horn with increased follicular activity and mean concentration of estradiol-17β in treated compared to non-treated buffaloes between days 14 and 21 postpartum. The earlier uterine involution and resumption of ovarian activity in the experimental group corresponded with significant (P>0.05) enhancement of progesterone at day 21 and 20% pregnant animals after hormonal treatment. In conclusion, the application of GnRH-PGF2α protocol 7 days apart starting at day 14 postpartum resulted in accelerated uterine involution and earlier resumption of the ovarian activity and could be used for improvement of the reproduction in postpartum Bulgarian Murrah buffaloes.
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