National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Phytase unavailability in monogastric livestocks leads to the release of undigested phytate phosphorus in their faeces and urine causing severe ecological problems. Phytic acid (or phytate) also acts as an anti-nutritional chelating agent for various metal ions, thus reducing the nutritive quality of food. Hence phytase supplementation can be used to reduce the environmental problem as well as improve the bioavailability of various minerals in food. In the present study efforts were made to isolate, screen and characterize efficient phytase producing strains of bacteria from different soil samples in Central Kerala, India. A total of 42 bacterial isolates were recovered and tested for their phytase activity and three of them, S13 (1), S13 (4) and S14 showed significant phytase activity on Phytase Screening Medium (PSM) agar plate. The isolates were initially identified by morphological and biochemical analysis. Amplification of 16SrRNA gene using specific primers, nucleotide homology analysis and phenotypic characterization revealed that these isolates showed homology to Bacillus sp, Pseudomonas sp. and Lysinibacillus sp. respectively. Optimization of culture conditions for maximum phytase production was also carried out. This is the first study that reports the production of phytase from Lysinibacillus sp. isolated from soil samples.
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