This study presents mass multiplication system through callus mediated organogenesis in popular Anthurium andreanum cv. Tropical Red, Acropolis and Esmeralda. Petiole with leaf (PL) and Leaf lamina with mid rib explants of all cultivars were sterilized, inoculated in callus induction and shoot induction medium (CISIM: MS salts, inositol 100 mg/l, thymine HCL 0.4mg/l, sucrose 30gm/l, 2, 4-D 0.2mg/l, BAP 1mg/l and agar 0.60gm/l) and incubated in dark. Creamy and compact callus initiated in cut ends along the veins of explants in 30-35 days of inoculation. The significantly highest callus induction response in both PL (94.45±9.45) and LLMR (74.67±5.89) explants was observed in cv. Esmeralda. Then, the callus was sub cultured at 30-35 days interval for 6 passages under dark incubation for maximization callus. The average callus multiplication ratio was 2.61-4.40 times in different cultivars. After sixth subculture, the callus was incubated in dark for 45 days for shoot bud initiation and elongation on CISIM. The number of shoot buds per explants varied with genotype, they were ranged from 30-39 per explants and 26-39 of them attained 1.5-2.5 cm in height. Then, These shoots were separated, inoculated in shoot elongation, multiplication cum rooting medium (SEMRM: MS salts, inositol 100 mg/l, thymine HCL 0.4mg/l, pyridoxine HCL 1mg/l, calcium pentothenate 1mg/l, Nicotinic acid 1mg/l, BAP 1.5mg/l, IAA 0.3mg/l) and incubated in light. Remaining callus after separation of shoots was sub cultured in CISIM and incubated in dark. This was continued for 6 passages for maximization of shoot culture. The significantly highest callus (2.31±0.24), shoot multiplication (2.46±0.29) ratio and average number of buds (21.11±2.31) was observed in cv. Tropical Red, respectively. The average callus and shoot multiplication ratio was 1.81-2.31 and 1.45-2.46 in different cultivars, respectively. The average shoot length was significantly highest in Anthurium cv. Acropolis Shoots attained 5.31-6.98cm in different cultivars in 45-55 days along with rooting. The present mass multiplication system is useful in production of large scale good quality planting material in the mention Anthurium cultivars.

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