National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The isolation of gold nanoparticles synthesizing bacteria from natural sources and their identification are very important in terms of discovering new industrial products. Keeping in view, local goldmine at Khaltunala and four hot water springs of Himachal Pradesh were selected as a source for new gold nanoparticles synthesizing bacteria. Therefore, aim of the present study was the isolation and characterization of gold nanoparticles synthesizing bacteria from these sites for synthesis of gold nanoparticles. Eighty five bacterial isolates were isolated from the samples using nutrient agar medium. All the bacterial isolates were studied for various morphological characters. Eighty five bacterial isolates were screened individually for their ability to synthesize gold nanoparticles and only 38 bacterial isolates, from goldmine were shown to possess gold nanoparticles synthesizing activity. Formation of gold nanoparticles was confirmed by colour change of the solution from yellow to purple/red wine colour. An indigenous bacterial isolate capable of gold nanoparticles synthesis was isolate and identified as a Bacillus licheniformis GPI-2, based on its morphology and analysis of its16S rDNA gene sequencing. After 6-12 hrs of incubation, monodisperse gold nanoparticles were, obtained. Which were characterized and found to be of 45 nm size. Gold nanoparticles were characterized through FTIR, transmission electron microscope with the size range of 40-45 nm. The process of synthesis of nanoparticles using an indigenous bacterial isolate from gold mine pebbles sample has been reported in this study, leading to the development of an easy bioprocess for synthesis of GNPs with many potential applications. The use of gold compounds and gold nanoparticles, with respect to their potential therapeutic applications such as anti-angiogenesis as anti-malarial agent, anti-arthritic agent, drug delivery, gene transfer, cancer nanotechnology, drug delivery applications, and its optical properties for cancer diagnosis and photo thermal therapy and biosensors, bio imaging, antimicrobial activity, food preservation.
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