National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The bioconversion of cellulose is important for global stabilization and a suitable human society. In this study, efficient cellulolytic bacteria were screened and isolated from paper industry effluent contaminated soil. Among the isolates, three strains showing higher potential for cellulase production were purified and sub cultured on carboxy methyl cellulose (CMC) agar plates. Among the three strains, one strain was given maximum enzyme activity. The isolate were identified as Bacillus subtilis sub.sps by morphological, physiological, biological and 16S rRNA gene sequence analysis. The isolated strain produced cellulase enzyme complex, with suggested synergic cellulolytic systems in Bacillus subtilis sub.sps. Cellulase was produced in cellulase enzyme production media containing waste paper as carbon source and the culture conditions like temperature, pH, incubation time and medium components and nitrogen sources were optimized. Optimal concentration of inoculum for enzyme production is 2% and incubation time is 60hrs. Optimum temperature and pH of the medium for the cellulase production by Bacillus subtilis sub.sps was 45°C and pH 6-7.
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