|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Phragmites australis, common pond reed with high lignocelluloses content is suitable for bioethanol production. In the present study pretreated pond reed was first fermented to sugars using cellulose and xylan degrading microorganisms isolated from cow dung and organic kitchen waste. Further fermentation of sugars to ethanol was performed using combinations of Sachharomyces cerevisae, Trichoderma reesei and Pichia stipitis. The isolated culture, O2.1 along with the combination of Trichoderma ressei and Pichia Stipitis exhibited maximum yield of ethanol (3.53gm %). Further identification of O2.1 was done by 16S-rDNA sequencing. The deduced sequence was used to retrieve the homologous sequences and to construct a phylogenetic tree. The results suggest the use of O2.1 for better ethanol production from pond reeds which can also help in value addition to the waste.