National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Success rate in selection of putative transformants is mainly dependent upon the optimum concentration of selective agent. Effect of antibiotic concentrations of hygromycin (10, 20, 30 and 50 mg/L) and paromomycin (10, 20, 30 and 50 mg/L) in callusing and regeneration media on immature embryo-derived calli was evaluated using wheat variety PBW621. Hygromycin at 10 mg/L and paromomycin at 15 mg/L gave about 50% regeneration from calli (compared to more than 90% in control) leading to identification of these concentrations as optimal. In a second experiment, seedlings derived from immature embryos were subjected to hygromycin, kanamycin and paromomycin at 5, 10, 15, 20 and 25 mg/L. Hygromycin and paromomycin gave 100 percent killing of seedlings at 20 and 25 mg/L concentrations, respectively, and were identified as optimal concentrations for screening of seedlings. Kanamycin treated seedlings turned albino even at lowest concentration of 5 mg/L. A similar response had been observed in case of calli, showing relative unsuitability of kanamycin as a selective agent.
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