National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The aim of the study is to isolate the bacteria and fungi causing keratitis in and around the Nellore district and to do antibiotic sensitivity testing especially to identify the MDR strains. Corneal scrapings were collected from 150 patients who attended to the department of Ophthalmology, Narayana Medical College, Nellore during the period February 2016 to December 2016 with corneal ulcers. A specific proforma was prepared to record the case history. After a detailed case history and clinical examination the corneal scrapings were collected by the ophthalmologist under slit – lamp examination. Three different samples were collected under strict aseptic precautions by using 26 gauge sterile needle, one for microscopy on Gram staining and KOH mount, another for culture and sensitivity on blood agar, chocolate agar and Mac Conkey’s agar for pyogenic bacteria and third sample for fungal culture on SDA medium. Out of 150 cases studied 82% (79) of cases belongs to 11-50 years age group. Among the study group 62.6% were males and 37.4% were females. 45.3% of agricultural workers and 13.3% of house wives were recognized as major suffers. 57.3% of corneal ulcer cases were found during October to December months. Corneal trauma accounted as major predisposing factor accounting for 78% of cases. Vegetable matter was responsible for corneal trauma in 39.3% of cases. Blood agar showed growth with 30 % (45) of cases chocolate agar with 29.3% (44) cases, Mac Conkey with 3.3% (5) cases and SDA with 13.3% (20) cases. 32 (21.3%) fungal isolates and 18 (12%) bacterial isolates were identified from the study group. Among the Gram positive bacteria Streptococcus pneumoniae 10(55.5%), Staphylococcus aureus 2 (11.1%) Pseudomonas aeruginosa 4 (22.2%) and Klebsiella 2 (11.1%) were identified. Fusarium spp. constitutes 46.8 % (15) Aspergillus spp. with 43.75% (14) and Curvularia 9.3 % (3) among the fungal isolates.
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