National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The biodynamic agriculture emphasizes the use of cowhorn silica manure (BD 501) for the improvement of growth and enhances quality and yield of crop plants. In this study, the microbial load in BD 501 manure was enumerated and evaluated for its antagonistic properties against selected rice pathogens. Three predominant bacterial isolates, BD (A)S1, BD(A)S2 and BD(A)S3 were isolated, purified, characterized and identified through microscopical, biochemical and 16S rDNA sequencing methods. These bacterial isolates were identified as Bacillus spp. based on the microscopic observation, Gram staining and biochemical tests. At species level, BD (A)S1 and BD(A)S2 isolates were identified as Bacillus amyloliquefaciens and isolate BD(A)S3 was identified as Bacillus toyonensis. These 3 isolates were tested for their antimicrobial activity against rice pathogens such as Rhizoctonia solani, Pyricularia oryzae and Xanthomonas oryzae, the causal organism of sheath blight, blast and bacterial blight of rice plants respectively. The bacterial isolate Bacillusamyloliquefaciensrecorded for a strong antifungal activity against R.solani and moderate activity against Pyricularia oryzae. This study provides a basis for confirming/ understanding the beneficial effect of BD 501.
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