National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
During survey from March 2014 to October 2015, in Barasat, West Bengal, India, incidence of symptoms suggestive of virus infection was observed in Coccinia grandis. Infected plants were showing typical symptoms including leaf curling, crinkled leaves, leaf yellowing and stunted growth. Symptomatic plant samples were tested for the presence of potyvirus and geminivirus. The occurrence of potyviruses was confirmed by ACP-ELISA, RT-PCR and nucleotide analysis of the part of genome. On the other hand, occurrences of geminiviruses were confirmed by southern blot analysis, polymerase chain reaction (PCR) and nucleotide analysis of the part of genome. Sequence analysis showed that the potyvirus from C. grandis shares 92% identity with Papaya ring spot virus (PRSV) isolate at the nucleotide level. The Geminivirus from the C. grandis shares 98 % identity with Tomato leaf curl New Delhi virus at the nucleotide level. This is the first evidence of mixed infection of Papaya ring spot virus and Tomato leaf curl New Delhi virus in C. grandis in India.
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