National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In the present study a total of ten Rhizobial bacterial isolates collected from different sources. These cultures were studied morphologically and biochemically for purity confirmation. The screening test results revealed that, among all Rhizobial isolates, GNR-1 showed best plant growth promoting abilities in in-vitro conditions. This promising culture was selected for main experiment toevaluate the shelf life of carrier based bio inoculants in different carrier materials stored at different temperatures in different intervals. The selected efficient PGP Rhizobium (GNR-1) and isolate was multiplied in large quantities in appropriate culture broth by incubating at 28±2°C in an incubator shaker at 120 rpm till they attained log phase with a cell load of l×l09cfu ml-1. For biofertilizer production Vermicompost, Vermiculate, Lignite, and Sodium Alginate carriers were used for bio inoculant preparation and stored at different temperatures i.e. 4ºC and 28ºC. Though the bacterial population decreased from 1st month to 8th month the survival rate of rhizobial cells was more upto 8th month at 40C. Similarly with respect to evaluation of shelf life of bacterial cells in different carrier materials like sodium alginate vermicompost lignite and vermiculite, sodium alginate based inoculants maintained good population count upto 8th month.
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