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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:6, June, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 3090-3097
DOI: https://doi.org/10.20546/ijcmas.2017.606.366


Cultural and Morphological Variability in Sclerotium rolfsii  Causing Stemrot Disease
V. Karthik Pandi1*, C. Gopalakrishnan2 and V. Janahiraman3
1Department of Plant pathology, TNAU, Coimbatore-641003, India
2Agricultural College and Research Institute, Kudumiyanmalai, India
3Department of Microbiology, Agricultural College and Research Institute, Madurai, India
*Corresponding author
Abstract:

Sunflower (Helianthus annus) is one of the important oil seed crops in India, which belongs to the family Asteraceae (Compositae). On the basis of cultural and morphological identification, 8 isolates of Sclerotium rolfsii were collected from different locations. Thus the present study was undertaken to know the pathogenicity and variability, so as to device better practices of the diseases to study cultural characteristic. In this study, eight isolates of stem rot were cultivated in Petri plates from sunflower, were established the following Kochs Postulates. The isolates were grown in PDA medium. Isolate SFSR1 in Petri plates recorded significantly maximum mycelial growth per day (31.45 mm) followed by SFSR3 and SFSR6. The isolate SFSR4 has recorded minimum mycelial growth per day (21.62 mm). In the production of sclerotia the isolate SFSR4 has produced significantly higher number of sclerotia (360 per plate) followed by SFSR1 (359.75), SFSR3 (324.25), SFSR6 (320.22), SFSR5 (290.68), SFSR2 (279.00) and SFSR8 (279.00). The isolate SFSR7 has produced minimum number of sclerotia (274 /plate). Among the isolates the biggest sclerotium (1224 mm) was produced by SFSR1 isolate followed by SFSR3 (1080 mm) and SFSR6 (1076 mm). The smallest sclerotium (1002 mm) was observed in SFSR8


Keywords: Helianthus annus, Sclerotium rolfsii, Stemrot disease, PDA medium

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How to cite this article:

Karthik pandi, V., C. Gopalakrishnan and Janahiraman, V. 2017. Cultural and Morphological Variability in Sclerotium rolfsii causing Stemrot Disease.Int.J.Curr.Microbiol.App.Sci. 6(6): 3090-3097. doi: https://doi.org/10.20546/ijcmas.2017.606.366
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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