National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Various extracts on the basis of polarity of solvent of Erythrina variegate L. (family- fabaceae) leaves such as hexane, chloroform, dichloromethane, ethyl acetate, methanol and aqueous were evaluated for their phytochemicals and antibacterial activity against selected five bacterial culture viz. Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris by disc diffusion method. The activities of the samples were compared with that of standard antibiotics e.g. Amikacin. Phyto-chemical screening of Erythrina variegata leaf extract in five different solvents showed the presence of important phyto-constituents like phenols, alkaloids, flavonoids, tannins and saponins. In antibacterial assay, all the organisms respond to the plant extract but inhibitory zone developed. Among the selected solvent used in soxhlet apparatus extract were hexane, chloroform, dichloromethane, ethyl acetate, methanol and aqueous, all developed inhibition zone against bacteria; this means selected plant has antibacterial property. The chloroform extract showed a higher activity than other extracts. The methanolic leaf extract of E. variegate showed higher activity against Pseudomonas aeruginosa and Staphylococcus aureus followed by Klebsiella pneumoniae, Proteus vulgaris and Escherichia coli. The methanolic leaf extract of E. variegata showed maximum zone of inhibition against Staphylococcus aureus. The ethyl acetate extract of E. variegata exhibited high activity against E. coli and Staphylococcus aureus followed by methanol, chloroform, hexane and aqueous extracts. The results revealed that the antimicrobial activity exhibited by E. variegata chloroform and aqueous extract noted to be most effective than other solvents.
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