National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A naturally occurring serum hemagglutinin was detected in the sand lobster, Thenus orientalis. The serum hemagglutinating activity was highest with buffalo erythrocytes. Preliminary characterization of the serum hemagglutinating activity showed that the activity was independent of calcium ions. However, there was reduced activity when exposed to divalent cationchelator, such as, EDTA and the activity was effectively restored with calcium ions. The HA activity of the lobster serum was stable between pH 7 and 9 and showed thermal stability between 20 and 40°C. Haemagglutination-inhibition assays performed with several carbohydrates revealed that the serum agglutinin was specific for N - acetylated amino sugars and that the presence of the N-acetylation was essential for agglutinin-ligand interaction. Also, the haemagglutinating activity of the serum could be specifically inhibited by lipopolysaccharides from various gram negative bacteria.
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