National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
This study aimed to molecular diagnosis of Klebsiella oxytoca using polymerase chain reaction. A total of 325 samples were collected from different clinical and environmental sources from six main hospitals in Baghdad province which is capital of Iraq. Out of 282 isolates, fifty six isolates (19.9 %) were isolated and identified as Klebsiella spp., forty isolates (12.3 %) were isolated and identified as K. pneumoniae (36 clinical and 4 environmental), ten isolates (3.5 %) were isolated and identified as Klebsiella terrigena (10 clinical only), three isolates (1.1 %) were isolated and identified as Klebsiella oxytoca and Klebsiella ornitholytica (2 clinical and 1 environmental) for each one. PCR was performed with the primer that target the pehX, Result showed that all the K. oxytoca isolate gave a clear band with a molecular weight 344 bp in size and this gene was not found in other Klebsiella spp. These results suggest that pehX genotype might be a useful marker to promote identification of K. oxytoca in Iraq, and used as routine protocol in public laboratories in Iraq.
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