National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Wilt caused by Fusarium oxysporium f. spciceris is a devastating disease of chickpea. It occurs in 2 stages; seedling stage (0-30%) and reproductive stage (0-57%). Annual chickpea yield loss due to Fusarium wilt was estimated to be 10% in India. So, our objective is to control chickpea wilt disease by using Trichoderma based biofilms as an alternative to chemical fungicides. Trichoderma viride is a potential antagonistic fungi which prevents diseases like wilt, brown rot, damping off, charcoal rot etc. We have isolated different strains of PGPR bacteria from waste lands of Parthenium rhizosphere soils to prepare a biofilm. A biofilm is an aggregate of microorganisms in which cells are stuck to each other and/or to biotic/abiotic surface. Our work was aimed towards the development of biofilms under in vitro conditions, using a combination of agriculturally important potential microorganisms like Bacillus subtilis, Pseudomonas flourescens and Rhizobium leguminosarum with the fungus Trichoderma viride as the matrix and screened for various biochemical traits like Antifungal activity, Ammonia production, HCN production, IAA production, Protein content, Siderophore production and Phosphate solubilization; and when compared to individual treatments, coinoculations and biofilms the biofilm performed well in all the biochemical properties. These biofilms were evaluated for their disease management and crop production in chickpea. A field experiment which comprised of 9 treatments were conducted. The synergism in terms of the PGP traits in the biofilms revealed their promise as superior PGP inoculants hence this in vitro experiment is to be carried out under field conditions to show better results.
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