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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:6, June, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 1619-1634
DOI: https://doi.org/10.20546/ijcmas.2017.606.190


Blast of Rice in Manipur and its Biocontrol by Pseudomonas fluorescens and Trichoderma sp.
T. Subhalakshmi* and S. Indira Devi
Microbial Resources Division, Institute of Bioresources and Sustainable Development, An autonomous DBT Research Institute, Government of India Takyelpat, Imphal- 795001, Manipur, India
*Corresponding author
Abstract:

Blast of rice (Oryza sativae L.) caused by Pyricularia oryzae is one of the most destructive disease in Manipur giving losses ranging from 60 to 100%. In the present investigation, biocontrol of this disease was attempted by isolating local strains of Pseudomonas fluorescens and Trichoderma spp. P. fluorescens B 24 gave maximum mycellial inhibition (77.5%) among the bacterial biocontrol and T. koningiopsis T 162 gave maximum inhibition of 46.25%. Seed germination, root and shoot length were enhanced by single treatment (B 24) in vitro conditions. Plant height was also increased by 5% in greenhouse and 12.02% in field trial with single application (B 24). However, greenhouse and field data revealed significant reduction in Blast incidence (5.1% and 3.4%), lesion number (35.53% and 58.72%) and size (18.86%, 16.39%) when applied in consortia (B 24+ T 162) as compared to single application (B 24) resulting in incidence of 6.7% and 3.92%, lesion number of 33.33% and 14.67%, size of 15.09% and 27.83% irrespective of greenhouse and field trial. The results indicated the effectiveness of combined application of P. fluorescens B 24 and T. koningiopsis T 162 for plant growth promotion and control of Blast of rice and therefore can be integrated for managing Blast of rice.


Keywords: Pseudomonas fluorescens, Trichoderma spp., Blast of rice, Biocontrol, Pyricularia oryzae,

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How to cite this article:

Subhalakshmi, T. and Indira Devi, S. 2017. Blast of Rice in Manipur and its Biocontrol by Pseudomonas fluorescens and Trichoderma sp.Int.J.Curr.Microbiol.App.Sci. 6(6): 1619-1634. doi: https://doi.org/10.20546/ijcmas.2017.606.190
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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