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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A total of seventy three NAB were isolated from surface sterilized nodules of different chickpea (Cicer arietinum) cultivars grown in the field from IARI, New Delhi using various kinds of media. Screening for the improved seedling growth showed that 91.78% of them enhanced radical length, and 87.67% of them increased plumule length under in-vitro conditions. About twenty four isolates were selected from preliminary screening and were subjected to their growth promoting potential under pot culture conditions using selected cultivar (PUSA 372). The promising nodule associated bacteria (NAB 69) showed a significant increase in plant growth regarding shoot dry weight (40.63%) and root dry weight (45.09%) in comparison to control. Its interactive effect was evaluated with Mesorhizobium ciceri for growth attributes and nodulation potential under pot culture conditions. The highest nitrogenase activity (74.12 μmoles C2H4/g of fresh weight of nodules/hr) was recorded in the treatment involving NAB 69 along with Mesorhizobium ciceri at 45 DAS (Vegetative stage). In-vitro screening for the functional potential of NAB showed a positive result with P solubilization. However, nitrogenase activity and siderophore production were not detected in the selected bacterial isolate. The 16S rDNA sequencing revealed that the bacterial isolate was closely related to Enterobacter sp. with 99% maximum identity. This investigation led to identifying the natural associations between the most promising nodule associated bacterium and Mesorhizobium ciceri in chickpea and the positive influence of their interactions.