National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Piglet diarrhoea caused by beta2 toxin producing Clostridium perfringens is a serious problem to piggeries throughout the world. The newly recognized Doom breed of Assam, India can survive under poor rearing condition and is generally found to be disease resistant. Beta2 toxin was earlier reported to be associated with necrotic enteritis in piglets of different breeds but its role has not so far been reported in Doom breed. Two cases of piglet diarrhoea in their post mortem analysis were found to be havingsevere lesions of necrotic enteritis in their intestine. Bacterial isolation followed by biochemical and molecular analysis confirmed theirassociation with C. perfringens Type A possessing beta2 gene. Further, to findout the total C. perfringens load under such severe condition, it was quantified by qPCR anda maximum of 2 x 106 fold incerease was observed compared to thereported load for healthy piglets.
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