National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Fungal endophytes are living inside plants without any harmful effects; the prospecting about them is increased day by day because they can produce bioactive compounds which can be used in different applications. Here in, the current study was aimed to isolate the endophytic fungi from the Ociumum basilicum (Al Rihan) plant as safe microorganisms and evaluate their antimicrobial activities. Altogether 150 segments of which 50 segments each from leaf, stem and flower tissues of the plant was screened for the enumeration of the endophytic fungi using the modified surface sterilization techniques. The fungal isolates were cultured in Potato Dextrose Agar (PDA) medium and the isolated fungi were identified on the basis of their colony characterization on PDA medium and morphological features. The cultures as well as the host plant parts were extracted by organic solvents (ethyl acetate and methanol). Antimicrobial activity investigation was carried out against Gram positive (Bacillus subtilis and Staphylococcus aureus) and Gram negative (Escherichia coli and Pseudomonas aeruginosa) bacteria and two fungal species (Candida albicans and Aspergillus niger) using the disc diffusion method. Results showed that a total of 8 endophytic fungi were recorded and morphologically identified from the different plant parts; 3 strains from the leaves, 3 from the stems and 2 from the flowers. Among them 6 belong to Hyphomycetes and 2 belong to Coelomycetes. The most potent antibacterial activity was obtained from the ethyl acetate extract of Chaetomium sp. followed by the ethyl acetate extract of Geotrichum sp. The tested fungi were less susceptible to the endophytic fungi except the ethyl acetate extracts from Alternaria sp., Geotrichum sp. and Curvularia sp., which exerted moderate activity towards A. niger and C. albicans.
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