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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Sorghum grain mold disease is a serious threat for sorghum cultivation. Being a complex disease involving many fungi, basic understanding of pathogenicity is essential. In the present investigation, 14 sorghum seed samples were tested by dry seed examination and blotter method for the presence of fungi. The results revealed prominent infection of Alternaria spp. (21%) and Fusarium spp. (23%). In seedling pathogenicity test Drechslera sp. reduced vigour index significantly by reducing shoot and root lengths. Phytotoxicity of selected fungi in detached leaf bioassay revealed toxicity to monocot leaves. The Curvularia sp., Drechslera sp., and Fusarium sp. induced necrosis in rice, sorghum and sugarcane leaves. Enzymatic potential of selected fungi was realised in plate assay. The Drechslera sp. and Fusarium sp. are prominent producers of enzymes essential for pathogenicity. The present work demonstrated wide occurrence of grain mold pathogens in sorghum seed samples and also assessed their pathogenicity. The detached leaf bioassay and enzyme plate assays were useful in the detection of virulence in fungal isolates.
Agrios, G. N. (2005). Plant Pathology. 5thedn. Elsevier Academic Press, USA. pp 922.
Ambekar, S. S., Kamatar, M. Y., Ganesamurthy, K., Ghorade, R. B., Saxena, U., Chand, P., Jadav, B. D., Das, I. K., Nageshwararao, T. G., Audilakshmi, S. and Seetharama, N. (2011). Genetic enhancement of sorghum (Sorghum bicolor (L) Moench) for grain mold resistance: II. Breeding for grain mold resistance. Crop Protection 30:759-764.https://doi.org/10.1016/j.cropro.2010.06.024
Barnett, H. L., and Hunter, B. B. (1972). Illustrated genera of imperfect fungi. 4thedn. Bargess Publishing Company, USA. pp 216.
Corallo, A. B., Del Palacio, A., Oliver, M., Tiscornia, S., Simoens, M., Cea, J., de Aurrecoechea, I., Martínez, I., Sanchez, A., Stewart, S. and Pan, D. (2023). Fusarium species and mycotoxins associated with sorghum grains in Uruguay. Toxins 15:484-495. https://doi.org/10.3390/toxins15080484
Duarte, M. D. L. R., and Archer, S. A. (2003). In vitro toxin production by Fusarium solani f. sp.piperis. Fitopatologia Brasileira 28:229-235.https://doi.org/10.1590/S0100-41582003000300002
Ellis, M. B. 1971. Dematiaceous hyphomycetes. Common wealth Mycological Institute, England. pp 608.
Esele, J. P., Frederiksen, R. A., and Miller, F. R. (1993). The association of genes controlling caryopsis traits with grain mold resistance in sorghum. Phytopathology 83:490-495. https://doi.org/10.1094/Phyto-83-490
Forbes, G. A., Bandyopadhyay, R., and Garcia, G. (1992). A review of sorghum grain mold. In: Sorghum and Millets Diseases-A Second World Review. International Crops Research Institute for the Semi-Arid Tropics, Patancheru, Andhra Pradesh, India, pp 265-272.
Haque, A. H. M. M., Akon, M. A. H., Islam, M. A., Khalequzzman, K. M., and Ali, M. A. (2007). Study of seed health, germination and seedling vigour of farmers produced rice seeds. International Journal of Sustainable Crop Production. 2:34-39.
International Seed Testing Association. (1985). International rules for seed testing. Rules 1985. Seed Science and Technology 13:299-513.
Kasana, R. C., Salwan, R., Dhar, H., Dutt, S., and Gulati, A. (2008). A rapid easy method for the detection of microbial cellulases on agar plates using Gram’s iodine. Current Microbiology 57:503-507..https://doi.org/10.1007/s00284-008-9276-8
Little, C. R., Perumal, R., Tesso, T., Prom, L. K., Odvody, G. N., and Magill, C. W. (2012). Sorghum pathology and biotechnology- A fungal disease perspective: Part I. Grain Mold, Head Smut, and Ergot. The European Journal of Plant Science and Biotechnology. 6:10-30.
Mielniczuk, E. and Skwarylo-Bednarz, B. (2020). Fusarium head blight, mycotoxins and strategies for their reduction. Agronomy 10:1-26. https://doi.org/10.3390/agronomy10040509
Navi, S. S., Bandyopadhyay, R., Hall, A. J., and Bramel-Cox, P. J. (1999). A pictorial guide for the identification of mold fungi on sorghum grain. Information Bulletin No.: 59.ICRISAT and Natural Resources Institute, United Kingdom. pp 118.
Neergaard P. (2005). Seed Pathology. Vol I, The MacMillan Press Ltd., London. pp 839.
Ojuri, O. T., Ezekiel, C. N., Sulyok, M., Ezeokoli, O. T., Oyedele, O. A., Ayeni, K. I., Nwangburuka, C. C., Elliott, C. T. and Krska R. (2018). Assessing the mycotoxicological risk from consumption of complementary foods by infants and young children in Nigeria. Food and Chemical Toxicology121:37-50. https://doi.org/10.1016/j.fct.2018.08.025
Ora, N., Faruq, A. N., Islam, M. T. Akhtar, N., and Rahman M. M. (2011). Detection and identification of seed-borne pathogens from some cultivated hybrid rice varieties in Bangladesh. Middle-East Journal of Scientific Research. 10:482-488.
Park, J., Kim, D. H., Moon, J. Y., An, J. A., Kim, Y. W., Chung, S. H., and Lee, C. (2018). Distribution analysis of twelve mycotoxins in corn and corn-derived products by LC-MS/MS to evaluate the Carry-Over ratio during wet-milling. Toxins. 10:1-15. https://doi.org/10.3390/toxins10080319
Prom, L. K. (2004). The effects of Fusarium thapsinum, Curvularia lunata and their combination on sorghum germination and seed mycoflora. Journal of New Seeds. 6: 39-49. https://doi.org/10.1300/J153v06n01_03
Prom, L. K. (2023). Frequency of isolation of four fungal species colonizing sorghum grain collected from six lines in an anthracnose-infected field. Journal of Agriculture and Crops. 9:137-140. https://doi.org/10.32861/jac.91.137.140
Prom, L. K. and Cuevas, H. E. (2023). Reaction of sorghum differentials to grain mold infection in Puerto Rico. American Journal of Plant Sciences. 14:1207-1213. https://doi.org/10.4236/ajps.2023.1411081
Prom, L. K., Isakeit, T., Cuevas, H., Erattaimuthu, S. R. and Jacobsen, R. (2021). Sorghum seed fungal community and their association with grain mold severity, seed weight, and germination rate. Journal of Agriculture and Crops 7:14-19. https://doi.org/10.32861/jac.71.14.19
Prom, L. K., Perumal, R., Cisse, N. and Little, C. R. (2014). Evaluation of selected sorghum lines and hybrids for resistance to grain mold and long smut fungi in Senegal, West Africa. Plant Health Progress15:74-77. https://doi.org/10.1094/PHP-RS-13-0128
Prom, L., Isakeit, T., Perumal, R., Erpelding, J. E., Rooney, W., and Magill, C. W. (2011). Evaluation of the Ugandan sorghum accessions for grain mold and anthracnose resistance. Crop Protection30:566-571. https://doi.org/10.1016/j.cropro.2010.12.025
Rychlik, M., Lepper, H., Weidner, C. and Asam, S. (2016). Risk evaluation of the Alternaria mycotoxin tenuazonic acid in foods for adults and infants and subsequent risk management. Food Control 68:181-185. https://doi.org/10.1016/j.foodcont.2016.03.035
Selle, P. H., Moss, A. F., Truong, H. H., Khoddami, A., Cadogan, D. J., Godwin, I. D. and Liu, S. Y. (2018). Outlook: Sorghum as a feed grain for Australian chicken-meat production. Animal Nutrition 4:17-30. https://doi.org/10.1016/j.aninu.2017.08.007
Ssepuuya, G., Poucke, C. V., Ediage, E. N., Mulholland, C., Tritscher, A., Verger, P., Kenny, M., Bessy, C., and Saeger, S. D. (2018). Mycotoxin contamination of sorghum and its contribution to human dietary exposure in four sub-Saharan countries. Food Additives and Contaminants: Part A35:1384-1393. https://doi.org/10.1080/19440049.2018.1461253
Thakur R. P., Rao, V. P., Agarkar, G. D., Solunke, R. B., Bhat, B., and Navi, S. S. (2006a). Variation in occurrence and severity of grain mold pathogens in India. Indian Phytopathology 59:410-416.
Thakur, R. P., Reddy, B. V. S., Indira, S., Rao V. P., Navi, S. S., Yang, X. B., and Ramesh, S. (2006b). Sorghum grain mold. Plant Pathology and Microbiology 72:1-32.
Tuppad, D. S., and Shishupala, S. (2014). Evaluation of endophytic fungi from Butea monosperma for antimicrobial and enzyme activity. Journal of Medicinal Plants Studies 2:38-45.
Vanara, F., Scarpino, V., and Blandino, M. (2018). Fumonisin distribution in maize dry-milling products and by-products: Impact of two industrial determination systems. Toxins10:1-15. https://doi.org/10.3390/toxins10090357
Vidhyasekaran, P., Borromeo, E. S., and Mew, T. W. (1986). Host-specific toxin production by Helminthosporium oryzae. Phytopathology 76:261-266. https://doi.org/10.1094/Phyto-76-261.
Yago, J. I., Roh, J., Bae, S., Yoon, Y., Kim, H., and Nam, M. (2011). The effect of seed-borne mycoflora from sorghum and foxtail millet seeds on germination and disease transmission. Mycobiology 39:206-218. https://doi.org/10.5941/MYCO.2011.39.3.206