National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
10 isolates of Trichoderma asperellum was used for characterization of secondary metabolites through gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) analysis to establish valid correlation between the production of antifungal metabolites and their bio-efficacy as BCAs. The investigation revealed that the culture filtrate of T. asperellum isolates were showed the presence of 673 secondary metabolites at different retention time with a range of 39 (Ta-20) to 101 (Ta-12) with GC-MS. Out 673 volatile metabolites, 55 metabolites were found to be most abundant from which seven metabolites from Ta-14 and Ta-20, six metabolites from Ta-8, Ta-17 and Ta-29, five metabolites from Ta-45, Ta-15, Ta-10 and Ta-12 and remaining three metabolites from Ta-2 isolate respectively. Further, the five isolates viz.,Ta-2, Ta-8, Ta-10, Ta-20 and Ta-45 were used for the LC-MS and study showed the presence of nine antifungal metabolites viz., Viridin, Viridiol, Butenolides, Harzianolides, Ferulic acid, Viridiofungin A, Cyclonerodiol, Massoilactone and Gliovirin. Hence, these isolates were produced highest number of major volatile and antimicrobial compounds. Therefore, these isolates viz., Ta-45, Ta-10, Ta-20, Ta-8, and Ta-2 were considered as high potential bio-control agents against Sclerotium rolfsii pathogens.
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