Decolourization of Spent Wash using Bacteria Pseudomonas putida
Ajad Patel
1,
Ranjan Singh1*, Abhishek Vashishtha
2, Soni Tiwari
1, Prabhash Kumar Pandey
4, Vijay Kumar Shukla
5, Laxmi Kant Pandey
6, Femina Sobin
7, Tanim Arpit Singh
8,Trashi Agrah Singh
9, Neeraj Khare
10, Shipra Singh
11, Rajeeva Gaur
1and Mahendra Kumar Gupta
12
1Department of Microbiology, Faculty of Science, Dr. Rammanohar Lohia Avadh University,
Ayodhya, Uttar Pradesh, India
2Department of Microbiology, Maharaja Ganga Singh University, Bikaner, Rajasthan, India.
3Department of Soil Science and Agriculture Chemistry, Institute of Agriculture Science, Banaras Hindu University, Varanasi, Uttar Pradesh, India
4Center for Advance Research, Faculty of Medicine, King George’s Medical University,
Lucknow, Uttar Pradesh, India
5Department of Biochemistry, Faculty of Science, Dr. Rammanohar Lohia Avadh University,
Ayodhya, Uttar Pradesh, India
6Department of Biotechnology, St. Aloysius College (Autonomous), Jabalpur, Madhya Pradesh, India
7Department of Botany and Microbiology, St. Aloysius College (Autonomous), Jabalpur, Madhya Pradesh, India.
8Maharaja Ranjit Singh College of Professional Sciences, Indore, Madhya Pradesh, India.
9Bhagyoday Tirth College of Paramedical Science, Sagar, Madhya Pradesh, India
10Institute of Allied Medical Science and Technology, NIET, NIMS University, Jaipur, Rajasthan, India
11Department of Microbiology, RRPG College, Amethi, U.P., India
12School of Studies in Botany, Jiwaji University, Gwalior, Madhya Pradesh, India
*Corresponding author
Abstract:
With extremely high BOD, COD, and other harmful organic and inorganic elements, spent wash is among the most complicated and time-consuming wastewaters. Because of the presence of several water soluble, colouring chemicals and recalcitrant primarily melanoidins, distillery effluent maintains a very dark brown hue even after anaerobic treatment. The aim of the study includes standardising several criteria for the extraction of melanoidin pigment as well as the isolation and identification of bacteria from natural sources. Methods: Pseudomonas putida, a melanoidin-decolorizing bacterium, was isolated in a lab setting using the serial dilution technique, and the culture conditions were improved at different incubation temperatures, times, pH levels, carbon sources, and nitrogen sources. On 120 hours, or the fifth day of culture, the optimal decolorization (86.05%) of melanoidins were accomplished at pH 5 and 37 °C. Based on the results of optimising the culture settings, it was discovered that the chosen bacterial strain needs 1.5 gm of extra carbon (Dextrose) and 1.5 gm of supplementary nitrogen (Ammonium sulphate) in order to decolorize. After bacterial treatment, dextrose and ammonium sulphate are supplied to the effluent, which is both cost- and environmentally-friendly. By using bacterial strain Pseudomonas putida, this method of biological treatment successfully decolorizes melanoidin from distillery effluent.
Keywords: Decolourization, spent wash, bacteria, culture conditions, fermentation, sugar plants
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How to cite this article:
Ajad Patel, Ranjan Singh, Abhishek Vashishtha, Soni Tiwari, Prabhash Kumar Pandey, Vijay Kumar Shukla, Laxmi Kant Pandey, Femina Sobin, Tanim Arpit Singh, Trashi Agrah Singh, Neeraj Khare, Shipra Singh, Rajeeva Gaur and Mahendra Kumar Gupta. 2023. Decolourization of Spent Wash using Bacteria
Pseudomonas putida.
Int.J.Curr.Microbiol.App.Sci. 12(10): 63-73. doi:
https://doi.org/10.20546/ijcmas.2023.1210.008
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