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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 12, Issue:8, August, 2023

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2023.12(8): 113-123
DOI: https://doi.org/10.20546/ijcmas.2023.1208.014


Isolation and Molecular Confirmation of Pseudomonas aeruginosa in Chicken meat, Mutton and Pork
Mangalagiri Swetcha1, S. Sureshkhannan1*, K. Porteen1 and S. Ezhilvelan2
1Department of Veterinary Public Health and Epidemiology,
2Department of Livestock Product Technology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007, Tamil Nadu, India
*Corresponding author
Abstract:

Pseudomonas aeruginosa is an opportunistic pathogen and is ubiquitous in nature and commonly found in soil, water, plants, and raw meat. It is major predominant spoilage bacteria in meat under refrigerated conditions. The study aimed to characterize the isolation, identification and molecular confirmation of Pseudomonas aeruginosa in Chicken meat, Mutton and Pork. Study area, sampling, isolation, identification and molecular confirmation of Pseudomonas aeruginosa. A total 210 (n = 35 from each of the species) aerobically stored and frozen meat samples were collected from retail markets and slaughter houses in and around Chennai, India and analyzed. Among them, 22 isolates were confirmed as Pseudomonas aeruginosa by conventional, culture, biochemical and PCR assay targeting 16 SrRNA sequencing and amplification of rpoBgene. In this research, we successfully studied the occurrence, isolation, identification and molecular confirmation of Pseudomonas aeruginosa isolated from Chicken meat, Mutton and pork using conventional techniques.


Keywords: Pseudomonas aeruginosa, 16 SrRNA, rpoB gene, spoilage bacteria

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How to cite this article:

Mangalagiri  Swetcha, S. Sureshkhannan, K. Porteen and Ezhilvelan, S. 2023. Isolation and Molecular Confirmation of Pseudomonas aeruginosa in Chicken meat, Mutton and Pork.Int.J.Curr.Microbiol.App.Sci. 12(8): 113-123. doi: https://doi.org/10.20546/ijcmas.2023.1208.014
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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