National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Hydrangea (Hortensia) is a highly popular ornamental plant for garden decoration, and now it is commercially produced for cut flower branches. The aim of this study was to set up a protocol for large scale for in vitro propagation of Hydrangea macrophylla, for this purpose two type of explant (shoot tip and node) were sterilized and the best percentage of survival explants (40%) was obtained when explants immersed for 20 min in 1.0 or 1.5 % NaOCl. For in vitro multiplication, Murashige and Skoog medium supplemented with 1.0 mg/l BA and 2.0 mg/l Kin was the best treatment on shoot number. Using GA3 at 1.0 mg/l was a marked increase in plant length when compared with zero level (control). During the rooting stage, the medium containing 4.0 mg/l IAA with charcoal gave the longest plantlet and the highest number of leaves, while NAA at 1.0 mg/l without charcoal gave the highest number of roots and the longest root was found at 2.0 mg/l IAA without charcoal. The longest plant (6.17 cm) was achieved when peat moss: per lite (2: 1 v/v) acclimatization mixture was used compared with other mixtures.
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