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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:10, October, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 4054-4066
DOI: https://doi.org/10.20546/ijcmas.2020.910.465


Isolation, Screening and Characterization of Silica Solubilizing Bacteria from Direct Sown Paddy (Oryza sativa L.) Growing Areas of Andhra Pradesh
S. Vinod Babu1*, A.Vijaya Gopal1, N. Trimurtulu1, G. Kishore Babu2 and B. Sree lakshmi3
1Department of Agricultural Microbiology, Advanced Post Graduate centre, ANGRAU, Lam, Guntur, India 2Department of Soil Science, 3Department of Plant Pathology, RARS, ANGRAU, Lam, Guntur, India
*Corresponding author
Abstract:

Rice (Oryza sativa L.) production in India is an important part of the national economy. India is the world's 2nd largest rice producer with approximately 43 Million hectares planted area, accounting for 22% of the world’s rice production. Silica as beneficial element has function for its cell strength in growth and development of paddy, which is accumulated 10% of its dry weight. In the present study was collected twenty (20) direct sown paddy rhizosphere soil samples from four districts of Andhra Pradesh and recorded their geographical position of collected soil samples. From that twenty eight (28) Silica Solubilizing Bacteria (SiSB) isolated by using magnesium trisilicate (0.25 %) as an insoluble source of silica in Bunt and Roviramedia. Silica solubilizing isolates coded based on their soil sample collected geographical position, recorded the morphological, cultural characteristics of SiSB. SiSB isolates screened based on their silica solubilization efficiency both qualitatively and quantitatively. Among all 28 SiSB isolates SiKPP-1 has the high ability to solubilize silica both qualitatively and quantitatively (12.41 mm & 2.16 ppm) followed by SiPYY-3 (12.02 mm & 2.12 ppm) and lowest was showed by SiAGG-1 (5.20 mm & 0.52 ppm). SiKPP-1 showed the positive for the Starch hydrolysis test, Indole production test, Catalase test, Oxidase test, Gelatine liquefaction test, Methyl red test, Vogues Proskauer test, Citrate Utilization test and Ammonia production test. SiKPP-1 showed the negative result for Hydrogen sulphide test.


Keywords: Rice (Oryza sativa L.), Rhizosphere, Silica Solubilizing Bacteria (SiSB) and Magnesium trisilicate (Mg2O8Si3)

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How to cite this article:

Vinod Babu, S., A. Vijaya Gopal, N. Trimurtulu, G. Kishore Babu and Sree Lakshmi, B. 2020. Isolation, Screening and Characterization of Silica Solubilizing Bacteria from Direct Sown Paddy (Oryza sativa L.) Growing Areas of Andhra Pradesh.Int.J.Curr.Microbiol.App.Sci. 9(10): 4054-4066. doi: https://doi.org/10.20546/ijcmas.2020.910.465
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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