National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present investigation was carried out to standardize the plant bio regulators for in vitro shoot proliferation of Curcuma longa L. Cv. Roma. The finger tip of the C. longa was taken as explants and cultured on MS media supplemented with different concentrations of plant bio-regulators. The result showed that the explants surface sterilized with 70% ethanol for 30 Sec, followed by 0.1% HgCl2 for 11 minutes, followed by 1% KCl washing for 1 min reduced the fungal %, bacterial % and death %. The similar treatment recorded highest survival (90%) and aseptic (90%), minimum days to bud emergence (11.31), maximum available days to bud elongation. MS media supplemented with BAP (3.0 mg/l), NAA (0.2 mg/l) and GA3 (0.2 mg/l) produces longer bud (0.7 cm). This protocol can be very useful to the future research worker and as well as entrepreneurs for mass production of Curcuma Longa L. Cv. Roma.
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