Isolation and Characterization of Brassicasterol from N-Hexane Fraction of Pometia pinnata and its Toxicity Test

Yoan De N; a Herru; Adlis Santoni; Mai Efdi

doi:https://doi.org/10.20546/ijcmas.2021.1009.024

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Original Research Articles Volume : 10, Issue:9, September, 2021

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
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Int.J.Curr.Microbiol.App.Sci.2021.10(9): 207-215

DOI: https://doi.org/10.20546/ijcmas.2021.1009.024

Isolation and Characterization of Brassicasterol from N-Hexane Fraction of Pometia pinnata and its Toxicity Test

Yoan De Nanda Herru, Adlis Santoni and Mai Efdi*

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Andalas University, Indonesia

^*Corresponding author

Abstract:

Pometia pinnata leaves were extracted and fractionated using n-hexane, dichloromethane, ethyl acetate, and methanol. The four fractions obtained were screened for cytotoxic testing using the Brine shrimp lethality test (BSLT) method, n-hexane fraction has the highest LC50 419,855 mg/L.The n-hexane fraction was continued for the isolation stage and a secondary metabolite compound was obtained, namely brassicasterol. The structure of this secondary metabolites was determined using spectroscopic methods (UV-Vis, FTIR, and NMR).

Keywords: Brassicasterol, brine shrimp lethality test (BSLT), Pometia pinnata

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How to cite this article:

Yoan De Nanda Herru, Adlis Santoni and Mai Efdi. 2021. Isolation and Characterization of Brassicasterol from N-Hexane Fraction of Pometia pinnata and its Toxicity Test.Int.J.Curr.Microbiol.App.Sci. 10(9): 207-215. doi: https://doi.org/10.20546/ijcmas.2021.1009.024

Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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Original Research Articles Volume : 10, Issue:9, September, 2021

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