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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
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Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 2799-2809
DOI: https://doi.org/10.20546/ijcrbp.2017.604.323


Effect of osmotic stress and seed priming on wheat seed germination traits
Navin Chander Gahtyari1*, J. P. Jaiswal1, Mohammed Talha1, Rakesh Choudhary1, Meenakshi Uniyal1 and Naveen Kumar2
1Department of Genetics and Plant Breeding, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand-263145, India 2Department of Molecular Biology and Genetic Engineering, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand – 263145, India
*Corresponding author
Abstract:

A study was undertaken to evaluate the effect of increasing osmotic stress on various germination traits along with the capability of hydropriming and hormonal priming to counteract it. Seeds of five wheat genotypes were tested at four different stress levels viz., 0 bar, -3 bar, -9 bar and -15 bar created using PEG-6000, either seed primed with water (hydropriming) or with GA3 (Gibberellic acid) @ 50 ppm in a completely randomized design with three replications. With increase in osmotic stress from 0 bar to -15 bar, germination traits like germination percentage (81.5% to 15%), plumule length (7.8 cm to 0.4 cm), number of lateral roots (5 to 1.9), fresh weight (124 mg to 77.4 mg) goes down whereas, dry weight (24.7 mg to 40.5 mg) goes up. At 0 and -3 bar GA3 treatment was more effective for enhancing germination percentage (11.5% and 9.5% increase over hydroprimed) whereas at higher stress levels i.e. -9 and -15 bar hydropriming was found to be better than GA3 treatment (8% and 6% increase over GA3). Average germination percentage at all stress levels revealed PBW 175 (55.00 %), C 306 (54.69 %) and MACS 6272 (53.13 %) as osmotic stress tolerant group and UP 2828 (50.63 %) and K 1016 (49.06 %) as osmotic stress susceptible group.


Keywords: Bars, genotypes, gibberellic acid, hydropriming, osmotic stress
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How to cite this article:

Navin Chander Gahtyari, J. P. Jaiswal, Mohammed Talha, Rakesh Choudhary, Meenakshi Uniyal and Naveen Kumar. 2017. Effect of osmotic stress and seed priming on wheat seed germination traits.Int.J.Curr.Microbiol.App.Sci. 6(4): 2799-2809. doi: https://doi.org/10.20546/ijcrbp.2017.604.323