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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 2477-2491
DOI: https://doi.org/10.20546/ijcmas.2017.604.289


Isolation, Screening and Optimization for Laccase production by Scytalidium lignicola pesante under submerged fermentation
Amanpreet K. Sidhu, Sonali B. Darade, Praneeta P. Bhavsar, Vishwas B. Gaikwad and Sucheta N. Patil*
Department of Biotechnology, K.T.H.M College, Nashik, Maharashtra,India, India
*Corresponding author
Abstract:

Twenty Five lignolytic fungi were isolated from decaying wood samples and were screened for the laccase production using guaiacol and ABTS as indicator compounds. The positive isolates were confirmed for the presence of laccase using Azure B and quantitative enzyme assay in presence of catalase using ABTS as substrate. Further laccase producing capacity of fungus was estimated in seven different media where Czapex Dox medium showed highest enzymatic activity of 17.2 U/ml. Effect of carbon and nitrogen sources, initial pH, inoculum size, incubation period, incubation temperature, inducer and inhibitor on laccase production was evaluated. The optimal pH, incubation temperature, incubation period and inoculum size for the laccase production in submerged culture were found to be pH 6, 30áµ’C, 7days and 2disc/100ml medium respectively. Sucrose and sodium nitrate were the most suitable carbon and nitrogen sources for laccase production. Out of the different inducers and inhibitors, the prominent induction was observed with 2mM CuSO4 and 0.1% MgSO4 while sodium azide and formaldehyde inhibited the laccase production almost 94% and 74% respectively. Under the optimal condition the maximum laccase activity was determined to be 28.2U/ml which is supposed to be 1.5 times higher than the unoptimized conditions.


Keywords: ABTS, Catalase, Guaiacol, Laccase, Media optimization, Scytilidium lignicola and Submerged fermentation
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How to cite this article:

Amanpreet K. Sidhu, Sonali B. Darade, Praneeta P. Bhavsar, Vishwas B. Gaikwad and Sucheta N. Patil. 2017. Isolation, Screening and Optimization for Laccase production by Scytalidium lignicola pesante under submerged fermentationInt.J.Curr.Microbiol.App.Sci. 6(4): 2477-2491. doi: https://doi.org/10.20546/ijcmas.2017.604.289