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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 2467-2476
DOI: https://doi.org/10.20546/ijcmas.2017.604.288


Isolation and PCR Amplification of E. coli from Freshwater Fish (Cirrhinus cirrhosis) and its PCR Amplification of SHV Gene
R. Meiyarasi1, T. Mohanapriya, B. Monika, M. Shravanthika, S. Nithyapriya, Jesteena Johney2 and R. Ragunathan*
1Department of Biotechnology, Kumaraguru College of Technology, Chinnavedampatti, Saravanampatti, Coimbatore-49, India
2Centre for Bioscience and Nanoscience Research, Eachanari, Coimbatore-21, India
*Corresponding author
Abstract:

Aquaculture products can harbor pathogenic bacteria which are part of the natural microflora of the environment. Feeding infected fish with antibiotic- medicated food is a general practice but has led to antibiotic resistance development in bacterial pathogen, resulting in a higher dose requirement for effective control, a matter of increasing public concern. Resistance of pathogens to antibiotics occurs due to the presence of β-lactamase enzyme, thereby resulting in Multiple Drug Resistance (MDR). These enzymes are present in Enterobacteriaceae such as E. coli. This study was conducted aiming at the isolation of E. coli from the intestines, muscle, head and tail of apparently healthy freshwater fish Cirrhinus cirrhosis collected from the local dam in Coimbatore district, Tamilnadu. Differentiation and characterization of E. coli isolates was based on their growth characteristics on specific culture media. E. coli was observed to be present in higher amounts in all parts of the fish. E. coli was identified and PCR amplification was performed for the isolated E.coli. The Hi Comb assay was also performed.


Keywords: Fish, E. coli, Multi Drug Resistance (MDR) SHVand PCR
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How to cite this article:

Meiyarasi, R., T. Mohanapriya, B. Monika, M. Shravanthika, S. Nithyapriya, Jesteena Johney and Ragunathan, R. 2017. Isolation and PCR Amplification of E. coli from Freshwater Fish (Cirrhinus cirrhosis) and its PCR Amplification of SHV Gene.Int.J.Curr.Microbiol.App.Sci. 6(4): 2467-2476. doi: https://doi.org/10.20546/ijcmas.2017.604.288