National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was conducted to evaluate the prevalence of Panton Valentine Leukocidin (pvl) in methicillin resistant S. aureus isolated from Chicken meat marketed in retail outlets in Chennai city, Tamil Nadu. A total of 120 meat samples were collected from different retail outlets and it was observed that 66.67 (80/120) per cent of the samples were positive for the presence of S. aureus. The isolates were screened for methicillin resistance phenotypically by methicillin, oxacillin and cefoxitin disc diffusion assay and for presence of mecA gene by PCR. The results revealed that 54 isolates were positive for presence of mecA gene by PCR indicating that the prevalence of methicillin resistant S. aureus (MRSA) was 67.5 per cent. Comparison of different disc diffusion assays with mecA PCR revealed that cefoxitin disc diffusion assay has sensitivity, specificity, Positive predictive value (PPV), Negative predicative value (NPV) and accuracy of100, 91, 100, 89 and 95 per cent respectively as compared to oxacillin and methicillin disc diffusion assay.Both MRSA and Methicillin susceptible S. aureus (MSSA) were screened by PCR for the presence of pvl gene and it was observed that 38 isolates carried pvl gene of which 28 isolates were MRSA and 10 isolates were MSSA indicating an overall prevalence of 47.5 per cent (38/80). The results of the present study indicates that MRSA isolated from retail chicken meat carries pvl geneclearly indicating the presence of Community associated-MRSA involving human contamination and hence proper hygiene is essential to prevent possible ill effects to the consumers.
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