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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 2568-2580
DOI: https://doi.org/10.20546/ijcmas.2017.604.299


Lactobacillus casei isolated from human milk and three natural agents act as antibacterial against gram negative pathogenic Bacteria isolated from infected eye
Enas Ibrahim Jasim, Basam Basim Al-Fraji* and Duaa Suhail Shawket
Department of Biology, Collage of Science, University of Al-Mustansiriyah, Baghdad, Iraq
*Corresponding author
Abstract:

One of the most common causes of eye infections is bacteria. The symptoms of these infections in humans can vary from mild to life-threatening complications. This study was conducted to determine the Gram negative bacterial pathogens that infect the eyes of people whether wearing lenses or not & investigate the inhibitory effect of some antibiotics and compare them with the antibacterial effect of black & green tea extracts , honey (at 10%) and CFS of Lactobacillus casei that isolated from milk of healthy mother against the pathogens isolated from the eye by using the agar diffusion technique. A total of 30 samples collected from infected eyes by swabs and transferred to the laboratory for microbial culturing. 20 different isolated species of Gram negative bacteria were identified by Vitek-2 system are (Enterobacter cloacae, Pseudomonas luteola, Leclercia adecarboxylata & pantoea agglomerans). All natural agents showed varying effectiveness against the bacteria isolated from the eye , while the most of isolates were resisted to antibiotics , Honey & CFS of Lactobacillus casei were most effective against growth of all the test organisms. Black and green tea showed less effect than honey & probiotic against bacteria. Our results showed that a lot of the commercial antibiotics were not effective on the tested organisms. Whereas the natural agents that used in this study had higher inhibitory effect against bacteria that isolates from ocular infections than the selected antibiotics .Further in vivo studies for the confirmation the antibacterial effect of natural agents that were used in this study.


Keywords: Lactobacillus casei, Breast milk, Probiotic, Enterobacter cloacae, Pseudomonas luteola, Leclercia adecarboxylata, Pantoea agglomerans, Camellia sinensis, Honey
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How to cite this article:

Enas Ibrahim Jasim, Basam Basim Al-Fraji and Duaa Suhail Shawket. 2017. Lactobacillus casei Isolated From Human Milk and Three Natural Agents Act as Antibacterial Against Gram Negative Pathogenic Bacteria Isolated From Infected Eye.Int.J.Curr.Microbiol.App.Sci. 6(4): 2568-2580. doi: https://doi.org/10.20546/ijcmas.2017.604.299