National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Drought tolerance is accompanied by number of traits and they are regulated by the many genes, hence targeting single gene against moisture stress will not be effective in crop improvement. Use of transcription factors (TFs) to target corresponding multigene is current trend. As per the many research findings, dehydration responsive element binding proteins (DREB) are reported as an important TFs that known to induces number of abiotic stress-related genes and impart the stress tolerance in plants. With this perspective, cotton variety Coker-312 was introduced with AtDREB1A gene and in the present study, transgenic cotton lines of T2 generation were evaluated for moisture stress resistance at Agriculture Research Station, Dharwad. Segregating T2 lines were screened with the help of PCR using gene specific primer and during moisture stress, DREB1A gene and its target gene fold change was studied using real time PCR (RT-PCR). Higher accumulation of the osmo-protectants like proline, reducing sugar was observed in the transgenic lines as compared to the non-transgenic lines under moisture stress.
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