National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A total seven soil samples were collected from the Shirala region Dist-Sangli. The total 70 Actinomycete isolates were obtained from soil by performing serial dilution technique and using Glycerol asparagine agar supplemented with Cycloheximide (100µg/ml). Actinomycete isolates were primarily identified to genus level on the basis of their microscopic and cultural characteristics. Biochemical and other characters were used to identify isolates to species level. A total 70 Actinomycete isolates were identified by using modified DOS based MICRO-IS software such as PIBWin. Out of 70 Actinomycete isolates total 82.85% isolates were belonged to genus Streptomyces, 10% isolates were belonged to genus Streptoverticillum, 2.8571% isolates were belonged to Nocardia, 2.8571% isolates were belonged to genus Micromonospora and rest of the 1.4285% isolate were belonged to Actinomadura. The primary screening of 70 isolates for tyrosinase production were carried out and isolates were showed positive tyrosinase activity.
Citations |
 |
 |
 |
 |
