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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 2153-2163
DOI: https://doi.org/10.20546/ijcmas.2017.604.253


Standardization of in-vitro Callus Induction and Regeneration Protocol for Mature Embryo of Proso Millet (Panicum miliaceum L.)
Bankar Ashish Haridas* and Gadakh Santosh Ashok
Department of Agricultural Botany, Dr. B.S.K.K.V., Dapoli-415412, India
*Corresponding author
Abstract:

The study was conducted to standardize an efficient protocol for callus induction, subsequent growth and in-vitro regeneration in six genotypes of proso millet viz., Gavhe, Sakhroli, Kalsuli, Vari No.10, Asond and Sukdhar. The seeds were surface sterilized with 70% ethanol for 1 minute + 1% HgCl2 for 10 minutes recorded maximum aseptic culture establishment in all genotypes. The mature embryo (seeds) of proso millet was cultured on MS medium supplemented with varying concentrations of 2,4-D (0.1-2.0 mg/l) and NAA (1.0-4.0 mg/l) NAA for callus induction for their callus induction and subsequent growth. Maximum frequency of callus induction was observed on MS media supplemented with 2 mg/l 2,4-D + 0.5 mg/l BAP. MS media supplemented with 2, 4-D produced friable, white and yellow colour calli while, in the same media supplemented with NAA produced loose light yellow and loose white colour calli. Maximum shoot regeneration was achieved by transferring calli on MS media supplemented with 4mg/l BAP and 4mg/l Kinetin. These regenerated plantlets were significantly showed rooting, when transferred on full strength MS supplemented with 0.2mg/l IBA + 0.1mg/l BAP and 2% sucrose. The rooted plantlet was hardened and transferred to soil.


Keywords: Mature embryo, MS medium, Callus induction, In vitro regeneration.
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How to cite this article:

Bankar Ashish Haridas and Gadakh Santosh Ashok. 2017. Standardization of in-vitro Callus Induction and Regeneration Protocol for Mature Embryo of Proso Millet (Panicum miliaceum L.).Int.J.Curr.Microbiol.App.Sci. 6(4): 2153-2163. doi: https://doi.org/10.20546/ijcmas.2017.604.253