National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In this study, we report adaptation of virulent field isolate of duck plague virus (DPV)/duck enteritis virus (DEV) in chicken embryo for the purpose of vaccine production. Naturally DEV field isolates are resistant to grow in chicken embryos and are not pathogenic to chicken embryo, unless few preliminary passages were given in embryonated duck eggs or duck embryo fibroblast cell culture. Historically, 12-20 number of duck embryo passages was reported to adapt the virus in chicken embryo. Here, we are first time reporting prerequisite of 50 number of serial passages in duck embryo to adapt the virus. The adapted virus killed the chicken embryo and was confirmed by PCR.
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