Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 2005-2020
DOI: https://doi.org/10.20546/ijcmas.2017.604.238


Lipase Production using Aspergillus japonicus MF-1 through Biotransformation of Agro-Waste and Medicinal Oil Effluent
Nathan Vinod Kumar1,2*, Mary Esther Rani1, R. Rathinasamy Gunaseeli3 and Narayanan Dhiraviam Kannan4

1Research Centre, Department of Botany and Microbiology, Lady Doak College, Madurai -625002, Tamil Nadu, India
2National Institute of Oceanography-CSIR, Regional Centre, Cochin 682018, Kerala, India
3Center for Environmental Studies, Lady Doak College, Madurai -625002, India
4Department Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai-62502i, Tamil Nadu, India
*Corresponding author
Abstract:

Oil rich effluents are always a menance for many industries in the process of effluent treatment. The process usually requires more complex methods to resolve the issue to reach to disposable effluent standards. Enzymatic degradation was proved to be promising for the efficient treatment of oil rich effluents. Among the hydrolytic enzymes, lipases are the second most widely-used enzymes in industrial applications. A potential lipolytic fungus obtained from a marine soil sample exposed to oil spillage was identified as Aspergillus japonicus MF-1. A maximum activity of 266 U/g was observed using solid state fermentation (SSF) utilizing groundnut oil cake. Lipase activity was found to be enhanced by oxidizing and reducing agents. Medicinal oil effluent was biotransformed to produce lipase under submerged fermentation to achieve a maximum activity of 153.4 U/ml. For lipase production using A. japonicus MF-1, solid state fermentation was found to be a cost effective and better strategy. Moreover, the usage of medicinal oil like effluents for lipase production is quite promising for bio-remediation of oil rich effluents.


Keywords: Lipase, Solid-state fermentation, Enzyme activity, Oil cakes, Oil effluent, Optimization.
Download this article as Download

How to cite this article:

Nathan Vinod Kumar, Mary Esther Rani, R. Rathinasamy Gunaseeli and Narayanan Dhiraviam Kannan. 2017. Lipase Production using Aspergillus japonicus MF-1 through Biotransformation of Agro-Waste and Medicinal Oil EffluentInt.J.Curr.Microbiol.App.Sci. 6(4): 2005-2020. doi: https://doi.org/10.20546/ijcmas.2017.604.238