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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 1759-1769
DOI: https://doi.org/10.20546/ijcmas.2017.604.211


Genetic Fidelity Analysis in the Micropropagated Banana Derived from Immature Primordial Male Flower Bud
N. Nandhakumar1, K. Soorianathasundaram1, D. Sudhakar2 and K.K. Kumar3*
1Department of Fruit Crops, Tamil Nadu Agricultural University, Coimbatore-641 003, India
 2Department of Plant Biotechnology, CPMB&B, Tamil Nadu Agricultural University, Coimbatore-641 003, India
3Plant Breeding and Genetics Agricultural College and Research Institute, Killikulam, Tuticorin District-628 252, India
*Corresponding author
Abstract:

Genetic uniformity of in vitro raised plants is a prerequisite for production of quality planting material of banana. Embryogenic cultures were induced from immature male flower buds of two important cultivars of banana, ‘Rasthali’ (Silk-Musa AAB) and ‘Grand Naine’ (Cavendish-Musa AAA). Cell suspensions were established and regenerated plants were evaluated for their genetic stability using 10 inter simple sequence repeat markers. ISSR marker produced totally 57 (Rasthali) and 64 (Grand Naine) clear, distinct and reproducible band classes resulting in a total of 1488 (Rasthali) and 1534 (Grand Naine) bands. The banding patterns for 10 primers were highly uniform and comparable to the field grown mother clone from which the cultures have been established. These results suggest that the establishment of embryogenic cell suspension cultures of banana could be an efficient system for production of genetically stable plants on a large scale as well as a biotechnological tool to support banana genetic improvement.


Keywords: Musa spp, Micropropagation, Somaclonal Variation, ISSR and Genetic Fidelity.
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How to cite this article:

Nandhakumar, N., K. Soorianathasundaram, D. Sudhakar and Kumar, K.K. 2017. Genetic Fidelity Analysis in the Micropropagated Banana Derived from Immature Primordial Male Flower Bud.Int.J.Curr.Microbiol.App.Sci. 6(4): 1759-1769. doi: https://doi.org/10.20546/ijcmas.2017.604.211