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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 1744-1752
DOI: https://doi.org/10.20546/ijcmas.2017.604.209


Analysis of Acetyl Co-A Carboxylase Activity in Marine Diatoms Isolated from Vellar Estuary, Southeast Coast of India
Shailesh Upadhyay1*, Kapil Lawrence1, Sudhir K. Shekhar2,M.  Suresh3 and P. Anantharaman3
1Department of Biochemistry and Biochemical Engineering, JIBB, Sam Higginbottom University of Agriculture, Technology and Sciences (SHUATS) (formerly Allahabad Agricultural Institute) Allahabad, 211007 India
2Department of Biotechnology, Babasaheb Bhimrao Ambedkar University (A Central University), Lucknow, India
 3CAS in Marine Biology, Annamalai University, Parangipettai, Tamilnadu, India
*Corresponding author
Abstract:

Certain strains of microalgae known as diatoms were shown, under appropriate conditions, to produce up to 60% of their cellular mass as lipids under certain growth conditions. Acetyl-Co-A carboxylase catalyses the first committed step in the synthesis of fatty acids, i.e., carboxylation of acetyl-CoA to malonyl-CoA. In present study we have isolated the two species of marine diatoms Odontella aurita and Chaetoceros muelleri from Vellar estuary Parangipettai Southest coast of India and studied the acetyl co-A carboxylase activity at different Si treatment nutrient medium. Results show that ACCase activity was maximum in treatment T2 (53 µM Si concentration) 0.035±0.006 U/ mg protein in O. aurita, 0.037±0.006 U/ mg protein in C. muelleri and minimum in treatment T4 (153 µM Si concentration) 0.019±0.001 U/ mg protein in O. aurita, 0.023±0.001 U/mg protein in C. muelleri. ACCase activity in treatment (T1) 0.031±0.0012 in O. aurita, 0.032±0.006 U/mg protein in C. muelleri while treatment (T3) show 0.024±0.001 in O. aurita, 0.026±0.001 U/ mg protein in C. muelleri. Treatment T2 show maximum ACCase activity so treatment (T2) further optimised. Optimum pH for ACCase activity was 8.0 and temperature 300C and 0.5 mM acetyl co-A. All treatments, acetyl co-A carboxylase activity slightly higher in C. muelleri than O. aurita.


Keywords: C. muelleri, O. aurita, Si Treatment, Acetyl co-A carboxylase
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How to cite this article:

Shailesh Upadhyay, Kapil Lawrence, Sudhir K. Shekhar, M. Suresh and P. Anantharaman. 2017. Analysis of Acetyl Co-A Carboxylase Activity in Marine Diatoms Isolated from Vellar Estuary, Southest Coast of India.Int.J.Curr.Microbiol.App.Sci. 6(4): 1744-1752. doi: https://doi.org/10.20546/ijcmas.2017.604.209