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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 1576-1587
DOI: https://doi.org/10.20546/ijcmas.2017.604.194


Isolation and In Silico Analysis of Iron Regulated Transporter 2 Gene from Indian Barnyard Millet (Echinochloa frumentacea L.)
T.K. Manape1, S. Varanavasiappan1, M. Raveendran1, S. Robin2,S.Rajeswari2 and D. Sudhakar1*
1Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India 2Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
*Corresponding author
Abstract:

Iron is one of the essential mineral for all organisms and its deficiency leads to many serious problems in human being worldwide. Plants have evolved with specific ZIP transporters to uptake and transport the iron from the soil. IRT2 is one of the ZIPs family members, which has a role in uptake of Fe2+ from the soil. Indian barnyard millet (E. frumentacea L.) is one of the rich sources of iron among millets. IRT2 gene of Indian barnyard millet is up regulated under iron deficient condition and hence we isolated EfIRT2 gene. In silico analysis of EfIRT2 gene showed that it codes for 386 amino acids, having molecular weight 40.54 kDa with pI 8.65. Its predicted structure showed that it is a plasma membrane protein with eight conserved domains and a signal peptide towards N-terminal end, with a Histidine signature of ZIPs family in between domain III and IV. Analysis based on deduced amino acid sequences of EfIRT2 showed highest similarity with SiIRT2 gene and clustered together with IRT2 proteins of Poaceae family members. Like other ZIPs family members, EfIRT2 could be a potential candidate gene for biofortification of staple foods.


Keywords: IRT2, barnyard millet, iron uptake, ZIP, in silico
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How to cite this article:

Manape, T.K., S. Varanavasiappan, M. Raveendran, S. Robin, S. Rajeswari and Sudhakar, D. 2017. Isolation and In Silico Analysis of Iron Regulated Transporter 2 Gene from Indian Barnyard Millet (Echinochloa frumentacea L.)Int.J.Curr.Microbiol.App.Sci. 6(4): 1576-1587. doi: https://doi.org/10.20546/ijcmas.2017.604.194