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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 1342-1348
DOI: https://doi.org/10.20546/ijcmas.2017.604.164


Prevalence of Metallo Beta Lactamase Production among Bacterial Isolates in Cases of Neonatal Septicemia in HAHC Hospital, Delhi, India
Mehvash Haider*, Parul Bansal, Ayan Kumar Das, Mridu Dudeja and Shyamasree Nandy
Department of Microbiology, Second Floor, HIMSR, JamiaHamdard, New Delhi, India
*Corresponding author
Abstract:

Neonatal septicemia is responsible for nearly half of neonatal deaths. With emerging drug resistance mechanisms like Metallo Beta lactamase (MBL) production, treatment of neonatal sepsis has become a challenge. The present study was done to identify prevalence of bacterial pathogens, their resistance profile and incidence of MBL mediated resistance among Gram negative bacterial isolates in neonatal sepsis. Seventy three cases of suspected neonatal septicemia were included in this prospective study. Blood culture was done by BacT/Alert 3D system, bacterial isolates were identified. All Imipenem-resistant isolates were screened for the production of MβL by the Imipenem-EDTA disc method and by Imipenem with and without EDTA (IPM+EDTA/IPM) Ezy MIC E Strips test. Of 73 blood samples, 42(57.5%) showed bacterial growth. Of those, 23 were Gram positive and 19 were Gram negative. The most common pathogen was Staphylococcus aureus. Most common Gram negative isolate was Acinetobacter spp (42%). 9(47.3%) Gram negative isolates were resistant to Imipenem. Of these, 8(88.8%) showed expanded growth inhibition zone when subjected to Imipenem-EDTA disk synergy (EDS) test indicating positive MBL production and were also positive for MBL production by Ezy MIC E Strips test. The present study indicated that although Gram-positive species continue to be the predominant causative organisms in neonatal sepsis, Gram negative bacteria have a special significance due to high prevalence of Carbapenem resistance and MBL production. High prevalence of drug resistance is a matter of concern as it leaves the physician no or few options for antimicrobial therapy.


Keywords: Metallo beta lactamase, Neonatal septicemia, Gram negative bacteria
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How to cite this article:

Mehvash Haider, Parul Bansal, Ayan Kumar Das, Mridu Dudeja and Shyamasree Nandy. 2017. Prevalence of Metallo Beta Lactamase Production among Bacterial Isolates in Cases of Neonatal Septicemia in HAHC Hospital, Delhi.Int.J.Curr.Microbiol.App.Sci. 6(4): 1342-1348. doi: https://doi.org/10.20546/ijcmas.2017.604.164