National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Epigenetic modulation plays a vital role in regulating the transcriptional machinery of human genome in response to infection. Differential Methylation pattern of the genome especially the retro transposon repetitive elements (RE) which constitutes more than 45% of the genome had been studied to trace the impact of RE methylation in the disease severity. The correlation of the levels of genome wide DNA methylation and the methylation levels of LINE1 (Long interspersed transposable elements), SINE- Alu (Short interspersed transposable elements) repetitive elements (RE) showed the influence of repetitive elements in the prognosis of Mycobacterium tuberculosis among pediatric population. In comparison with healthy controls in this study we have investigated the association of methylation status altering the transcriptome involved in eliciting immune response. Investigating the levels of methylation of the repetitive elements will be an index to analyze the genome wide methylation which implicates the severity of the disease. Genome wide Methylation profiling could also be a potential biomarker to correlate with the prognosis of tuberculosis. The global methylation status of children infected with TB was found to be significantly differentiated when compared with healthy children. The methylation pattern of the repetitive elements LINE1&SINE (Alu) were also found to be significantly differentiated with that of controls. Significant genome wide hypomethylation was observed in children infected with TB (9% (IQR, 7%-10%) in comparison with healthy children (22 % (IQR, 18%-24%) (P<0.001). The repetitive elements LINE1 and SINE (Alu) in accordance with global methylation of the genome were found to be hypomethylated in children infected with TB. The percentage of LINE1 Methylation was 22% (IQR, 20%-26%) in cases and 67% (IQR 62-70%) in controls(P<0.0001) respectively. Similarly, remarkable difference in the levels of SINE(Alu) Methylation was found between cases (27% (IQR 24-30%) and controls 65% (IQR 58-68%) (P <0.0001)
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