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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:4, April, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 1267-1273
DOI: https://doi.org/10.20546/ijcmas.2017.604.154


Comparative Evaluation of c-ELISA and i-ELISA for Detection of Bluetongue Virus Antibodies in Sheep
S.S. Patel*, H.C. Chauhan, B.S. Chandel, K.B. Patel, M.D. Shrimali, A.C. Patel,  Manish Rajgor, J.K. Kala,  M.G. Patel, M.A. Patel and B.K. Patel
Department of Animal Biotechnology and Veterinary Microbiology, Veterinary College, S.D.A.U., Sardarkrushinagar-385506, Gujarat (India)
*Corresponding author
Abstract:

Bluetongue (BT) is an infectious, non-contagious disease of domestic and wild ruminants. Bluetongue virus (BTV) causes severe disease in sheep, which is transmitted by insect vector belonging to Culicoides spp. It is particularly a viral disease of sheep, occasionally affecting cattle, buffaloes, goats, camels and other wild ruminants. In present study, out of 382 sheep serum samples, 150 (39.26%) and 130 (34.03%) were positive respectively by c-ELISA and i-ELISA. On comparing relative sensitivity and specificity of i-ELISA to c-ELISA was 86.67 and 100.00 per cent, respectively. Overall agreement between both the tests was 94.76 per cent.


Keywords: Sheep, Bluetongue, c-ELISA, i-ELISA, Seroprevalance.

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How to cite this article:

Patel, S.S., H.C. Chauhan, B.S. Chandel, K.B. Patel, M.D. Shrimali, A.C. Patel, Manish Rajgor, J.K. Kala, M.G. Patel, M.A. Patel and Patel, B.K. 2017. Comparative Evaluation of c-ELISA and i-ELISA for Detection of Bluetongue Virus Antibodies in Sheep.Int.J.Curr.Microbiol.App.Sci. 6(4): 1267-1273. doi: https://doi.org/10.20546/ijcmas.2017.604.154
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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